- Cavitation food and stuffing it with Directed and Experimental Evolutionary soft robotics
- Famine by design in "Nothing from Nature" UN Mandate
- Transfecting plants [and humans] using a mosaic viral vector
- Florescent and barcoded magnofection (Cellular glowing magnetic food)
- By the end of 2021 commercial food supply will be mutated [edited] food.
Our food supply has been violently disrupted.
Three years ago, the United Nations (UN) put out a little-known report stating that soon food would be sold for 1/1000th of a calorie and if Defense Advanced Research Project Agency (DARPA) had their way it would be sold by the molecule. Link to State of Global Food Security and Nutrition 2019. My coverage on this topic can be found here: Link
One goal of the UN Sustainable Goals is to transform the entire food supply so that nothing is taken from nature. All consumables must be created in a laboratory, then recycled using the Cradle to Cradle scheme.
In 2020, we all noticed the COVID-19 weapon was interrupting the food supply chain. Crops and vegetables are a significant source of food, materials, and medicine. People were being told that biotechnological solutions were necessary for achieving food security.
This year the World Economic Forum announced that the entire commercial food supply would be genetically edited, bringing it into compliance with UN standards.
Ironically, the world is turning to genetically engineered crops to combat engineered supply chain shortages stemming from a biological weapon unleashed upon the people of the world.
To generate genetically engineered plants, the gene-of-interest edits must be efficiently delivered into the body cavity of cells or target their cellular organs - with minimal biological perturbations.
Traditional Plant Transfection Methods
One of the most commonly used tools for gene delivery in plants is Agrobacterium, a Gram-negative bacterium that can transfect both plant cells without cell walls and tissues such as leaves. As we recently have learned in my coverage of Kingdom Wars, integration of DNA cargo requires repeated and tedious plant transformation known as Directed Evolution. This process may randomly integrate with DNA, and thereby disrupt internal gene expressions. Most notably, Agrobacterium has also been reported to cause plant tissue necrosis - death.
The other popular technique used is biolistic particle delivery or gene gun. This method utilizes high energy to deliver biomolecule-coated gold particles directly into plant cells through physical disruption of the plant cell wall and membrane. As you might expect, this particle-bombardment severely injures the plant or can stifle the payload significantly the process of Directed Evolution.
You probably are aware of viral vectors as a platform system used in various jabs. Geneware technology uses a mosaic virus-based delivery system. Viral vectors are unable to deliver proteins directly, which makes them incompatible with popular gene editing techniques like CRISPR. It is claimed that viral vectors are subject to strict regulations due to their pathogenic origins and capabilities to integrate a portion of their genetic elements into the plant host genome - but that is nonsense these days where biotechnology supersedes law or regulation.
One emerging tool in the biotech toolkit is the use of magnetic iron-oxide nanoparticles bonded with DNA. This method is challenging to adapt to plant engineering. Scientists can overcome barriers using fluorescently-labeled and bar-coded magnetic nanoparticles as carriers to deliver genes through these cell-wall-free apertures. Magnetic fields are then applied to provide directional forces to transport the nanoparticle gene carriers into pollens. Using several types of imaging devices, scientists can track the florescence and energy until they make it to their final destination. Using the same polymerase chain reaction (PCR) test you are required to use - that results in a Darwinian Directed Evolution mutagenesis - they were able to successfully integrate transgenic characteristics to the genome, including successful inheritance to offspring. Magnetofection works in many plant species including pumpkin, lily, and cotton.
Pollen is an attractive biological target to transfect because pollen grains carry the male gametes, and so could generate a complete genetically modified plant after fertilization in the plant ovules. Scientists are really excited about magnetofection because it allows for them to avoid using time-consuming tissue culture based on regenerable tissues. Regeneration is feasible but technically very challenging for both sorghum and maize, monocot species. However, magnetofection is likely not a simple, universal pollen transfection method.
Carbon Nanotube Transfection
Research is demonstrating that aside from the use of magnetic nanoparticles, carbon nanotubes are also an increasingly popular nano-tool for plant transfection. Carbon nanotubes rely on passive delivery as a plant transformation tool. Research teams physically adsorb and electrostatically graft DNA encoding using green fluorescent protein (GFP) onto single-walled carbon nanotubes and showed that their technique can effectively deliver DNA into the leaves of a variety of plant species including wheat, cotton, and arugula for transient gene expressions. Carbon nanotube technology did not result in significant toxicity in mature leaves by performing quantitative PCR analysis of NbrbohB, a known stress gene.
A new species is engineered when isolates from arugula leaves were able to internalize carbon nanotubes and stably expressed the GFP plasmid with 80% inheritable transfer.
siRNA (si=small interfering RNA) is a useful material for transient plant genetic engineering. Using single-walled carbon nanotubes demonstrated that transfection of siRNA to inhibit and silence expressions of GFP in the leaves of tobacco plant occurred for up to a week. siRNA is biocompatible for use in plants.
The impact of using nanoparticles for plant genetic engineering is that delivery of DNA and RNA can be accomplished without pathogens ballistic force that disrupts the plant tissue. siRNA is considered to be a green alternative to the use of chemical pesticides, or the delivery of mRNA for DNA-free genetic manipulation of plants.
Nanotechnology has significantly enhanced genetic engineering and reprogramming of humans, animals, and plants. The first problem is that while it is relatively easy to engineer human and animals, plant cells have rigid cell walls which exclude large particles. It is unknown whether nanoparticles are able to diffuse easily through the multi-layered cell wall and then into the cell membrane. Complex biological differences among plant species and the types of biological materials from plants (leaves, pollens, and regenerable tissues like root hairs) mean that systematic characterization of plant-nanoparticle interactions are needed to enhance transfection outcomes.
Another problem relates to the size of the DNA cargo that can be delivered into plant cells, which is directly correlated to the surface area of the nanoparticles. Thus far, only small spherical nanoparticles and single-walled cylindrical carbon nanotubes have shown utility to transfect plant cells through passive diffusion and/or with the use of mechanical aid such as a gene gun. Unfortunately, this means that only small gene cargo load with potential for plant damage.
It has been shown that nanoparticles like carbon nanotubes can adversely inhibiting the growth of tomato and lettuce with some cell toxcitity after nanoparticle transfection.
Finally, most of the nanotechnology techniques for plant transfection offers transient gene expression. Long-term reproducible "phenotypes" [mutations] are necessary for bringing transfected crops to the commercial market.
Plant genetic engineering will become increasingly important because of growing population and increased demand for food, medicine, and materials. High-tech collaborators aim to energize the plant transformation toolkit using nanotechnology to generate positive societal impact.
We all must be ever vigilant in our food purchases. With some foods you will be able to visibly see technology at work, but most will remain invisible to the naked eye. The safest thing we can all do is grow our own food. We should also be diligent about blessing our food and asking the Lord to cover it and to protect us from unwittingly eating corrupted foods.
May you be blessed with pure food and nutrition,
Celeste Solum is a broadcaster, author, former government, organic farmer and is trained in nursing and environmental medicine. Celeste chronicles the space and earth conditions that trigger the rise and fall of modern & ancient civilizations, calendars, and volatile economies. Cycles are converging, all pointing to a cataclysmic period between 2020 to 2050 in what many scientists believe is an Extinction Level Event.
Tracking goods and people will be a part of managing the population during this convergence.
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